SF3B2

Protein-coding gene in the species Homo sapiens
SF3B2
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

2DO5

Identifiers
AliasesSF3B2, Cus1, SAP145, SF3B145, SF3b1, SF3b150, splicing factor 3b subunit 2, CFM
External IDsOMIM: 605591; MGI: 2441856; HomoloGene: 6678; GeneCards: SF3B2; OMA:SF3B2 - orthologs
Gene location (Human)
Chromosome 11 (human)
Chr.Chromosome 11 (human)[1]
Chromosome 11 (human)
Genomic location for SF3B2
Genomic location for SF3B2
Band11q13.1Start66,050,729 bp[1]
End66,069,308 bp[1]
Gene location (Mouse)
Chromosome 19 (mouse)
Chr.Chromosome 19 (mouse)[2]
Chromosome 19 (mouse)
Genomic location for SF3B2
Genomic location for SF3B2
Band19|19 AStart5,323,960 bp[2]
End5,345,483 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • ventricular zone

  • left testis

  • right testis

  • anterior pituitary

  • right uterine tube

  • ganglionic eminence

  • right lobe of thyroid gland

  • left lobe of thyroid gland

  • right hemisphere of cerebellum

  • body of uterus
Top expressed in
  • Rostral migratory stream

  • external carotid artery

  • internal carotid artery

  • supraoptic nucleus

  • condyle

  • conjunctival fornix

  • fossa

  • substantia nigra

  • motor neuron

  • primitive streak
More reference expression data
BioGPS
n/a
Gene ontology
Molecular function
  • protein binding
  • RNA binding
Cellular component
  • catalytic step 2 spliceosome
  • spliceosomal complex
  • U2-type spliceosomal complex
  • U12-type spliceosomal complex
  • precatalytic spliceosome
  • nucleus
  • U2 snRNP
  • nucleoplasm
  • nuclear speck
  • U2-type precatalytic spliceosome
Biological process
  • mRNA splicing, via spliceosome
  • mRNA processing
  • viral process
  • RNA splicing
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

10992

319322

Ensembl

ENSG00000087365

ENSMUSG00000024853

UniProt

Q13435

Q3UJB0

RefSeq (mRNA)

NM_006842

NM_030109
NM_001362454

RefSeq (protein)

NP_006833

NP_084385
NP_001349383

Location (UCSC)Chr 11: 66.05 – 66.07 MbChr 19: 5.32 – 5.35 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Splicing factor 3B subunit 2 is a protein that in humans is encoded by the SF3B2 gene.[5][6]

Function

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.[6]

Interactions

SF3B2 has been shown to interact with SF3B4,[7][8] RBM7,[9] SF3B1[10][11] and CDC5L.[12]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000087365 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000024853 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Gozani O, Feld R, Reed R (Jan 1996). "Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A". Genes & Development. 10 (2): 233–43. doi:10.1101/gad.10.2.233. PMID 8566756.
  6. ^ a b "Entrez Gene: SF3B2 splicing factor 3b, subunit 2, 145kDa".
  7. ^ Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
  8. ^ Champion-Arnaud P, Reed R (Aug 1994). "The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site". Genes & Development. 8 (16): 1974–83. doi:10.1101/gad.8.16.1974. PMID 7958871.
  9. ^ Guo TB, Boros LG, Chan KC, Hikim AP, Hudson AP, Swerdloff RS, Mitchell AP, Salameh WA (2003). "Spermatogenetic expression of RNA-binding motif protein 7, a protein that interacts with splicing factors". Journal of Andrology. 24 (2): 204–14. doi:10.1002/j.1939-4640.2003.tb02664.x. PMID 12634307.
  10. ^ Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R (Oct 1999). "Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155". Molecular and Cellular Biology. 19 (10): 6796–802. doi:10.1128/mcb.19.10.6796. PMC 84676. PMID 10490618.
  11. ^ Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R (Sep 2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". The EMBO Journal. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC 126279. PMID 12234937.
  12. ^ Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (Dec 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC 305846. PMID 11101529.

Further reading

  • Agell N, Aligué R, Alemany V, Castro A, Jaime M, Pujol MJ, Rius E, Serratosa J, Taulés M, Bachs O (1998). "New nuclear functions for calmodulin". Cell Calcium. 23 (2–3): 115–21. doi:10.1016/S0143-4160(98)90109-9. PMID 9601606.
  • Champion-Arnaud P, Reed R (Aug 1994). "The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site". Genes & Development. 8 (16): 1974–83. doi:10.1101/gad.8.16.1974. PMID 7958871.
  • Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M (Sep 1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nature Genetics. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529. S2CID 585778.
  • Das BK, Xia L, Palandjian L, Gozani O, Chyung Y, Reed R (Oct 1999). "Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155". Molecular and Cellular Biology. 19 (10): 6796–802. doi:10.1128/mcb.19.10.6796. PMC 84676. PMID 10490618.
  • Das R, Zhou Z, Reed R (May 2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E". Molecular Cell. 5 (5): 779–87. doi:10.1016/S1097-2765(00)80318-4. PMID 10882114.
  • Bryant HE, Wadd SE, Lamond AI, Silverstein SJ, Clements JB (May 2001). "Herpes simplex virus IE63 (ICP27) protein interacts with spliceosome-associated protein 145 and inhibits splicing prior to the first catalytic step". Journal of Virology. 75 (9): 4376–85. doi:10.1128/JVI.75.9.4376-4385.2001. PMC 114182. PMID 11287586.
  • Brand M, Moggs JG, Oulad-Abdelghani M, Lejeune F, Dilworth FJ, Stevenin J, Almouzni G, Tora L (Jun 2001). "UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation". The EMBO Journal. 20 (12): 3187–96. doi:10.1093/emboj/20.12.3187. PMC 150203. PMID 11406595.
  • Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, Lührmann R, Query CC (Aug 2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site". The EMBO Journal. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMC 125580. PMID 11500380.
  • Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ (Apr 2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
  • Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R (Sep 2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". The EMBO Journal. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC 126279. PMID 12234937.
  • Warner DR, Roberts EA, Greene RM, Pisano MM (Dec 2003). "Identification of novel Smad binding proteins". Biochemical and Biophysical Research Communications. 312 (4): 1185–90. doi:10.1016/j.bbrc.2003.11.049. PMID 14651998.
  • Will CL, Schneider C, Hossbach M, Urlaub H, Rauhut R, Elbashir S, Tuschl T, Lührmann R (Jun 2004). "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome". RNA. 10 (6): 929–41. doi:10.1261/rna.7320604. PMC 1370585. PMID 15146077.
  • Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
  • Lin KT, Lu RM, Tarn WY (Oct 2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo". Molecular and Cellular Biology. 24 (20): 9176–85. doi:10.1128/MCB.24.20.9176-9185.2004. PMC 517884. PMID 15456888.
  • Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (Jan 2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
  • Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
  • Ardon O, Zimmerman ES, Andersen JL, DeHart JL, Blackett J, Planelles V (Apr 2006). "Induction of G2 arrest and binding to cyclophilin A are independent phenotypes of human immunodeficiency virus type 1 Vpr". Journal of Virology. 80 (8): 3694–700. doi:10.1128/JVI.80.8.3694-3700.2006. PMC 1440437. PMID 16571786.
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  • 2do5: Solution structure of the SAP domain of human splicing factor 3B subunit 2
    2do5: Solution structure of the SAP domain of human splicing factor 3B subunit 2