SF3B14

Protein-coding gene in the species Homo sapiens

SF3B6

Available structures

PDB

Ortholog search: PDBe RCSB

List of PDB id codes
2F9D, 2F9J, 2FHO, 3LQV

Identifiers

Aliases

SF3B6, HSPC175, Ht006, P14, SAP14, SAP14a, SF3B14, SF3B14a, CGI-110, splicing factor 3b subunit 6

External IDs

OMIM: 607835; MGI: 1913305; HomoloGene: 9360; GeneCards: SF3B6; OMA:SF3B6 - orthologs

Gene location (Human)

Chr.	Chromosome 2 (human)^[1]

Band	2p23.3	Start	24,067,586 bp^[1]
Band	2p23.3	End	24,076,373 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 12 (mouse)^[2]

Band	12\|12 A1.1	Start	4,867,544 bp^[2]
Band	12\|12 A1.1	End	4,877,659 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

skin of arm

mucosa of ileum

olfactory zone of nasal mucosa

Achilles tendon

mucosa of sigmoid colon

ganglionic eminence

cartilage tissue

islet of Langerhans

rectum

tibialis anterior muscle

Top expressed in

saccule

otic placode

otic vesicle

medial ganglionic eminence

transitional epithelium of urinary bladder

migratory enteric neural crest cell

abdominal wall

cervix

endocardial cushion

ventricular zone

More reference expression data

BioGPS

n/a

Gene ontology
Molecular function	nucleic acid binding RNA binding protein binding mRNA binding
Cellular component	catalytic step 2 spliceosome U12-type spliceosomal complex U2-type spliceosomal complex precatalytic spliceosome nucleus U2 snRNP nucleoplasm spliceosomal complex
Biological process	mRNA splicing, via spliceosome mRNA processing blastocyst formation RNA splicing
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


51639


66055

Ensembl


ENSG00000115128


ENSMUSG00000037361

UniProt


Q9Y3B4


P59708

RefSeq (mRNA)


NM_016047


NM_025323

RefSeq (protein)


NP_057131


NP_079599

Location (UCSC)

Chr 2: 24.07 – 24.08 Mb

Chr 12: 4.87 – 4.88 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Splicing factor 3B, 14 kDa subunit, also known as SF3B14, is a human gene.^[5]

Function

This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.^[5]

Interactions

SF3B14 has been shown to interact with SF3B1.^[6]^[7]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000115128 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000037361 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ^a ^b "Entrez Gene: SF3B14 splicing factor 3B, 14 kDa subunit".
^ Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, Lührmann R, Query CC (August 2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site". EMBO J. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMC 125580. PMID 11500380.
^ Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R (September 2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". EMBO J. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC 126279. PMID 12234937.

Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W (2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". Genome Res. 10 (5): 703–13. doi:10.1101/gr.10.5.703. PMC 310876. PMID 10810093.
Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL (2000). "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning". Proc. Natl. Acad. Sci. U.S.A. 97 (17): 9543–8. Bibcode:2000PNAS...97.9543H. doi:10.1073/pnas.160270997. PMC 16901. PMID 10931946.
Zhang QH, Ye M, Wu XY, Ren SX, Zhao M, Zhao CJ, Fu G, Shen Y, Fan HY, Lu G, Zhong M, Xu XR, Han ZG, Zhang JW, Tao J, Huang QH, Zhou J, Hu GX, Gu J, Chen SJ, Chen Z (2000). "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells". Genome Res. 10 (10): 1546–60. doi:10.1101/gr.140200. PMC 310934. PMID 11042152.
Harrington JJ, Sherf B, Rundlett S, Jackson PD, Perry R, Cain S, Leventhal C, Thornton M, Ramachandran R, Whittington J, Lerner L, Costanzo D, McElligott K, Boozer S, Mays R, Smith E, Veloso N, Klika A, Hess J, Cothren K, Lo K, Offenbacher J, Danzig J, Ducar M (2001). "Creation of genome-wide protein expression libraries using random activation of gene expression". Nat. Biotechnol. 19 (5): 440–5. doi:10.1038/88107. PMID 11329013. S2CID 25064683.
Will CL, Schneider C, MacMillan AM, Katopodis NF, Neubauer G, Wilm M, Lührmann R, Query CC (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site". EMBO J. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMC 125580. PMID 11500380.
Will CL, Urlaub H, Achsel T, Gentzel M, Wilm M, Lührmann R (2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". EMBO J. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC 126279. PMID 12234937.
Golas MM, Sander B, Will CL, Lührmann R, Stark H (2003). "Molecular architecture of the multiprotein splicing factor SF3b". Science. 300 (5621): 980–4. Bibcode:2003Sci...300..980G. doi:10.1126/science.1084155. PMID 12738865. S2CID 31046270.
Will CL, Schneider C, Hossbach M, Urlaub H, Rauhut R, Elbashir S, Tuschl T, Lührmann R (2004). "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome". RNA. 10 (6): 929–41. doi:10.1261/rna.7320604. PMC 1370585. PMID 15146077.
Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.

PDB gallery

2f9d: 2.5 angstrom resolution structure of the spliceosomal protein p14 bound to region of SF3b155
2f9j: 3.0 angstrom resolution structure of a Y22M mutant of the spliceosomal protein p14 bound to a region of SF3b155
2fho: NMR solution structure of the human spliceosomal protein complex p14-SF3b155