PPP1R8

Enzyme found in humans

PPP1R8

Available structures

PDB

Ortholog search: PDBe RCSB

List of PDB id codes
3V4Y

Identifiers

Aliases

PPP1R8, ARD-1, ARD1, NIPP-1, NIPP1, PRO2047, protein phosphatase 1 regulatory subunit 8

External IDs

OMIM: 602636; MGI: 2140494; HomoloGene: 8555; GeneCards: PPP1R8; OMA:PPP1R8 - orthologs

Gene location (Human)

Chr.	Chromosome 1 (human)^[1]

Band	1p35.3	Start	27,830,782 bp^[1]
Band	1p35.3	End	27,851,676 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 4 (mouse)^[2]

Band	4\|4 D2.3	Start	132,554,240 bp^[2]
Band	4\|4 D2.3	End	132,570,480 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

Achilles tendon

ventricular zone

islet of Langerhans

ganglionic eminence

stromal cell of endometrium

smooth muscle tissue

lymph node

C1 segment

granulocyte

mucosa of sigmoid colon

Top expressed in

somite

mandibular prominence

maxillary prominence

epiblast

medial ganglionic eminence

abdominal wall

hand

otic vesicle

condyle

human fetus

More reference expression data

BioGPS


More reference expression data

Gene ontology
Molecular function	protein serine/threonine phosphatase inhibitor activity DNA binding protein binding RNA binding protein phosphatase inhibitor activity nuclease activity endonuclease activity ribonuclease E activity hydrolase activity mRNA binding protein phosphatase regulator activity
Cellular component	cytoplasm nuclear speck nucleoplasm spliceosomal complex nucleus
Biological process	regulation of transcription, DNA-templated nucleic acid phosphodiester bond hydrolysis mRNA processing RNA catabolic process transcription, DNA-templated RNA splicing cell population proliferation RNA phosphodiester bond hydrolysis negative regulation of protein dephosphorylation negative regulation of phosphoprotein phosphatase activity regulation of phosphoprotein phosphatase activity production of miRNAs involved in gene silencing by miRNA
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


5511


100336

Ensembl


ENSG00000117751


ENSMUSG00000028882

UniProt


Q12972


Q8R3G1

RefSeq (mRNA)


NM_138558 NM_002713 NM_014110


NM_001290725 NM_146154 NM_001355198 NM_001355199

RefSeq (protein)


NP_002704 NP_054829 NP_612568


NP_001277654 NP_666266 NP_001342127 NP_001342128

Location (UCSC)

Chr 1: 27.83 – 27.85 Mb

Chr 4: 132.55 – 132.57 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Nuclear inhibitor of protein phosphatase 1 is an enzyme that in humans is encoded by the PPP1R8 gene.^[5]^[6]^[7]

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.^[7]

Interactions

PPP1R8 has been shown to interact with PPP1CA,^[8]^[9] Histone deacetylase 2,^[8] SF3B1^[10] EED^[8] and the EZH2 domain of PRC2.^[11]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000117751 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000028882 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Wang M, Cohen SN (November 1994). "ard-1: a human gene that reverses the effects of temperature-sensitive and deletion mutations in the Escherichia coli rne gene and encodes an activity producing RNase E-like cleavages". Proc Natl Acad Sci USA. 91 (22): 10591–5. Bibcode:1994PNAS...9110591W. doi:10.1073/pnas.91.22.10591. PMC 45067. PMID 7524097.
^ Mishima K, Tsuchiya M, Nightingale MS, Moss J, Vaughan M (May 1993). "ARD 1, a 64-kDa guanine nucleotide-binding protein with a carboxyl-terminal ADP-ribosylation factor domain". J Biol Chem. 268 (12): 8801–7. doi:10.1016/S0021-9258(18)52945-8. PMID 8473324.
^ ^a ^b "Entrez Gene: PPP1R8 protein phosphatase 1, regulatory (inhibitor) subunit 8".
^ ^a ^b ^c Jin Q, van Eynde Aleyde, Beullens Monique, Roy Nivedita, Thiel Gerald, Stalmans Willy, Bollen Mathieu (August 2003). "The protein phosphatase-1 (PP1) regulator, nuclear inhibitor of PP1 (NIPP1), interacts with the polycomb group protein, embryonic ectoderm development (EED), and functions as a transcriptional repressor". J. Biol. Chem. 278 (33): 30677–85. doi:10.1074/jbc.M302273200. ISSN 0021-9258. PMID 12788942.
^ Ajuh PM, Browne G J, Hawkes N A, Cohen P T, Roberts S G, Lamond A I (February 2000). "Association of a protein phosphatase 1 activity with the human factor C1 (HCF) complex". Nucleic Acids Res. 28 (3): 678–86. doi:10.1093/nar/28.3.678. PMC 102561. PMID 10637318.
^ Boudrez A, Beullens Monique, Waelkens Etienne, Stalmans Willy, Bollen Mathieu (August 2002). "Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1". J. Biol. Chem. 277 (35): 31834–41. doi:10.1074/jbc.M204427200. ISSN 0021-9258. PMID 12105215.
^ Ferreira M, Verbinnen I, Fardilha M, Van Eynde A, Bollen M (November 2018). "The deletion of the protein phosphatase 1 regulator NIPP1 in testis causes hyperphosphorylation and degradation of the histone methyltransferase EZH2". J. Biol. Chem. 293 (47): 18031–18039. doi:10.1074/jbc.AC118.005577. PMC 6254331. PMID 30305391.

Van Eynde A, Wera S, Beullens M, et al. (1996). "Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing". J. Biol. Chem. 270 (47): 28068–74. doi:10.1074/jbc.270.47.28068. PMID 7499293.
Claverie-Martin F, Wang M, Cohen SN (1997). "ARD-1 cDNA from human cells encodes a site-specific single-strand endoribonuclease that functionally resembles Escherichia coli RNase E." J. Biol. Chem. 272 (21): 13823–8. doi:10.1074/jbc.272.21.13823. PMID 9153239.
Vulsteke V, Beullens M, Waelkens E, et al. (1998). "Properties and phosphorylation sites of baculovirus-expressed nuclear inhibitor of protein phosphatase-1 (NIPP-1)". J. Biol. Chem. 272 (52): 32972–8. doi:10.1074/jbc.272.52.32972. PMID 9407077.
Van Eynde A, Pérez-Callejón E, Schoenmakers E, et al. (1999). "Organization and alternate splice products of the gene encoding nuclear inhibitor of protein phosphatase-1 (NIPP-1)". Eur. J. Biochem. 261 (1): 291–300. doi:10.1046/j.1432-1327.1999.00272.x. PMID 10103062.
Jin Q, Beullens M, Jagiello I, et al. (1999). "Mapping of the RNA-binding and endoribonuclease domains of NIPP1, a nuclear targeting subunit of protein phosphatase 1". Biochem. J. 342. ( Pt 1) (1): 13–9. doi:10.1042/0264-6021:3420013. PMC 1220430. PMID 10432294.
Chang AC, Sohlberg B, Trinkle-Mulcahy L, et al. (2000). "Alternative splicing regulates the production of ARD-1 endoribonuclease and NIPP-1, an inhibitor of protein phosphatase-1, as isoforms encoded by the same gene". Gene. 240 (1): 45–55. doi:10.1016/S0378-1119(99)00435-7. PMID 10564811.
Boudrez A, Beullens M, Groenen P, et al. (2000). "NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry". J. Biol. Chem. 275 (33): 25411–7. doi:10.1074/jbc.M001676200. PMID 10827081.
Jagiello I, Van Eynde A, Vulsteke V, et al. (2001). "Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1". J. Cell Sci. 113. Pt 21 (21): 3761–8. doi:10.1242/jcs.113.21.3761. PMID 11034904.
Beullens M, Vulsteke V, Van Eynde A, et al. (2001). "The C-terminus of NIPP1 (nuclear inhibitor of protein phosphatase-1) contains a novel binding site for protein phosphatase-1 that is controlled by tyrosine phosphorylation and RNA binding". Biochem. J. 352. Pt 3 (Pt 3): 651–8. PMC 1221501. PMID 11104670.
Trinkle-Mulcahy L, Sleeman JE, Lamond AI (2002). "Dynamic targeting of protein phosphatase 1 within the nuclei of living mammalian cells". J. Cell Sci. 114 (Pt 23): 4219–28. doi:10.1242/jcs.114.23.4219. PMID 11739654.
Beullens M, Bollen M (2002). "The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly". J. Biol. Chem. 277 (22): 19855–60. doi:10.1074/jbc.M200847200. PMID 11909864.
Boudrez A, Beullens M, Waelkens E, et al. (2002). "Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1". J. Biol. Chem. 277 (35): 31834–41. doi:10.1074/jbc.M204427200. PMID 12105215.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Ammosova T, Jerebtsova M, Beullens M, et al. (2003). "Nuclear protein phosphatase-1 regulates HIV-1 transcription". J. Biol. Chem. 278 (34): 32189–94. doi:10.1074/jbc.M300521200. PMID 12788939.
Jin Q, van Eynde A, Beullens M, et al. (2003). "The protein phosphatase-1 (PP1) regulator, nuclear inhibitor of PP1 (NIPP1), interacts with the polycomb group protein, embryonic ectoderm development (EED), and functions as a transcriptional repressor". J. Biol. Chem. 278 (33): 30677–85. doi:10.1074/jbc.M302273200. PMID 12788942.
Vulsteke V, Beullens M, Boudrez A, et al. (2004). "Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1". J. Biol. Chem. 279 (10): 8642–7. doi:10.1074/jbc.M311466200. PMID 14699119.
Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.